Villa captain Chester represented Wales during the international break.
Chester, 29, was withdrawn after 50 minutes of Wales' UEFA Nations League loss to Denmark, in which Gareth Bale's late goal stood only as a consolation after Nicolai Jorgensen and Martin Braithwaite netted for Age Hareide's side.
It is believed that the former West Bromwich Albion and Hull City defender was carrying a knock prior to the clash anyway, but Ryan Giggs decided to start the defender anyway.
Birmingham Live report that he is now a doubt for Sunday's clash at Villa Park against bitter rivals Birmingham, who are in good form after winning four of their last six Championship outings under Garry Monk.
Dean Smith hardly has any options at centre-back, with Chester and Axel Tuanzebe partnering each other for every single minute of his five games in charge since replacing Steve Bruce.
Alan Hutton and Mile Jedinak have been used at centre-back, as has youngster James Bree this season, but against enemies Birmingham, fans will want to see a full-strength side in a bid to win the bragging rights.
Villa fans reacted on Twitter by slamming Wales for playing Chester with a prior knock, and are concerned that he will now be unavailable for Sunday's clash.
Time to call back Tommy Elphick pic.twitter.com/7jhEJtXIag— Cathal Galvin (@c_galvin64) November 20, 2018
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