The Saints took a huge step in their aim of winning their first trophy since 1976 thanks to a 2-0 win over Arsenal at the Emirates Stadium.
It means they will face Liverpool in a two-legged affair in January for a place at Wembley against either Manchester United or Hull City.
Puel made eight changes to the side that overcame Everton at the weekend and was rewarded as they were comfortable winners against Arsene Wenger's side.
The Saints boss is quoted in the Daily Echo, saying: “I don’t know my first team. Since the beginning of the season I am lucky because we can have different players for every position and very good players.
“I don’t want to say that yes this 11 players is my first team and the others the second team. We have many very good players."
Rotation from Puel is not limited to the EFL Cup as he has been juggling the Premier League and Europa League commitments as well; even if he did omit Jose Fonte, Oriol Romeu, Nathan Redmond and Charlie Austin completely from the squad for the game against Arsenal.
Puel is obviously encouraged by the resources currently at his disposal, especially given the manner of Southampton's victory, and the busy fixture period will continue so rotating his side around the time of the semi-final is inevitabile.
There are seven games for the Saints to deal with this month alone, and their participation in the FA Cup also gets underway in January.
While some will view Puel as being happy to chop and change his team, the Saints boss is clearly pleased with the competition for places, and feels he has enough to compete in as many competitions as possible.