The Scottish Championship winners crashed to a 3-2 defeat to Alan Stubbs' men.
Mark Warburton's men went into the game having already wrapped up the Scottish Championship title as well as having beaten their Old Firm rivals Celtic in the semi-final of the Scottish Cup this weekend.
Despite their tremendous achievements however, the Gers put in one of their most disappointing performances of the campaign as Alan Stubbs' side came out 3-2 winners with only a late Barrie McKay wonder-strike - his second in as many games - making the scoreline look slightly more respectable.
But despite none of their remaining league games holding any importance in terms of implications regarding promotion, Holt has dismissed the claim that the Light Blues were experiencing some kind of hangover from their semi-final and title-winning heroics.
"No not at all [there was no physical hangover]. You saw in the last ten minutes that boys were making the runs they were in the first ten minutes of the game so that certainly wasn't a problem," he told the Evening Times.
"I would disagree massively [that the motivation is missing]. Since the league is won, we are not a squad or group of boys to take our foot off the gas.
"If anything, we will be working even harder because we want to finish the season on a high."
While the midfielder has insisted that the squad had no problem preparing themselves for the game, the fact that they were guilty of considerable mistakes for all three of Hibs' goals would suggest that their minds were not quite on the job at hand.
But with the defeat doing no harm to their league position, Warburton may look back on the loss as an important one.
The game, against the side they will face in the Scottish Cup final next month, will remind the players that while they knocked the Scottish Premiership champions out in the last four at Hampden Park, they cannot assume that the domestic treble is in the bag.