The 25-year-old could be sidelined for up to two months.
The Reds' captain suffered ligament damage in Thursday night's 1-1 draw at Borussia Dortmund in the Europa League in a setback which is likely to rule him out for six to eight weeks, the club confirmed on Friday.
Consequently, the injury has also plunged Henderson's hopes of playing for England at Euro 2016 into major doubt, and legendary goalkeeper Clemence was quick to send his commiserations.
Jurgen Klopp's side had little to play for domestically, with their Champions League qualification hopes all but gone, though Henderson's layoff will certainly come as a blow with regard to winning the Europa League.
The Merseyside outfit have a big chance of reaching the semi-finals after Divock Origi netted a potentially vital away goal against Dortmund - the bookmakers' favourites - at Signal Iduna Park.
Joe Allen, who has to his credit looked a better player since Klopp replaced Brendan Rodgers in October, is expected to deputise.