The 23-year-old was replaced by Fraser Forster in first-half stoppage time after initially attempting to play on after appearing to suffer an injury before being beaten from long range by Toni Kroos' shot.
Butland's problem did not look good, and the former Birmingham City man has now revealed that he has suffered a fracture in his ankle.
He tweeted: "Today's scan revealed I've fractured my ankle, absolutely devasted (sic)! I'll be doing everything I can to get back fit as soon as I can!"
Today's scan revealed I've fractured my ankle, absolutely devasted! I'll be doing everything I can to get back fit as soon as I can!— Jack Butland (@JackButland_One) March 27, 2016
The injury is expected to rule the goalkeeper out for up to three months, which would see him miss the rest of the domestic season and be likely to miss the entirety of Euro 2016.
The news is a huge blow for the stopper, who won just his fourth senior cap for the Three Lions in the first half of the clash with the world champions in Berlin.
It will also come as massive setback for Stoke and any aspirations the club might have of getting up into the European position in the Premier League table, with Butland having been in fine form throughout the season.
Unsurprisingly, there was an outpouring of sympathy for the young goalkeeper, who has seen his hopes of playing at a first major tournament dashed.
@JackButland_One get well soon Jack. Hope you have a speedy recovery...— David Séadon (@DavidSeadon) March 27, 2016
@JackButland_One gutted for you Jack. Try to stay positive and remember all the good things this season.— StokieUK (@pharbron) March 27, 2016
@JackButland_One and when you are back fit Jack, just wait til you hear the ovation you'll get at The Brit. Chin up, and come back stronger— DUCK MAGAZINE (@DUCKmagstoke) March 27, 2016
@JackButland_One Absolutely devastated for you having to miss France but you've got an incredible career ahead of you still ⚪️— Mike Bell (@Mikestoke) March 27, 2016
@JackButland_One gutted for you Jack keep your spirits up, you've been outstanding this season. We'll be with you.— Dave Jones (@Bonner_DJ) March 27, 2016
@JackButland_One Gutted mate. One the best keepers around at the minute.deserved going to the Euros KRO mate ⚪️ U be back as good as ever!— Orbry (@orbry79) March 27, 2016