The Scottish Championship leaders face Dundee for a place in the last four of the competition.
The Light Blues are currently one short of the landmark, but despite coming up against Premiership opposition at the weekend, Holt is confident that they can bring up the century, and celebrate the achievement by booking their place in the semi-final of the competition.
"Hopefully we can score our 100th goal this weekend. We'll go into the match confident," he told the Record.
"In recent weeks we've managed to do the job of sending the fans home happy and we'll be looking to do the same on Saturday."
If the Gers are able to get on the scoresheet against Paul Hartley's men - which seems probable given the fact that they have only been denied on one occasion this term which came during their stalemate with Kilmarnock in February - then it would have taken them just 38 matches to reach the total, which would mean that Mark Warburton's side have averaged nearly three goals a game.
And if they can maintain that kind of scoring rate from now until the end of the season, they will surely have no problem running even further away with the Championship title, and will perhaps be serious contenders to win their 34th Scottish Cup.