The absence of key midfielders and a rotation in full-backs did not help matters.
Spurs needed a win against West Ham to go top of the table, however found themselves wanting against their London rivals.
A goal from the in form Michail Antonio proved enough for The Hammers to pick up a win against Spurs.
Tottenham are therefore three points behind Leicester City in the Premier League title race - with the small task of a North London derby to navigate this weekend.
The result was certainly a blow to their title hopes and one former Spurs player highlighted two reasons why they failed to pick up the result against The Hammers.
Danny Murphy spent one, rather unsuccessful, season at White Hart Lane. He believes the absence of two key midfielders and the full-back choices for the game were key in Spurs' defeat last night.
Speaking on Match Of The Day he stated:
"They were missing Dembele and Dele Alli was on the bench. That lost them the legs and the energy that we normally see.
"Also, for me personally, the two full-backs of Walker and Rose give them a bit more attacking threat than the two they had in tonight.
"Overall, disappointing. But it is only their first defeat on the road since the opening day of the season so it is not the end of the world."
Belgian Dembele is currently out injured whilst Dele Alli is carrying an ankle niggle. Meanwhile the full-backs at Spurs have been regularly rotated this season. But last night Ben Davies and Kiern Trippier did not step up in the absence of Rose and Walker - which did not help the Spurs cause.
Hopefully Alli, Walker and Rose will be fit and firing for the Arsenal game this weekend.