The Argentine is unsure if the youngster will face West Ham.
Before Sunday's 2-1 win at home to Swansea City, the 19-year-old prompted concern after appearing to suffer an ankle injury in shooting practice prior to kickoff.
The hugely-rated midfielder, who played the full 90 minutes that day, then sparked more fears over his fitness after being seen limping on to the team coach afterwards, plunging his participation at Upton Park tomorrow night into doubt.
Pochettino's side, who sit second in the Premier League table, two points behind Leicester City, host title rivals Arsenal at White Hart Lane on Saturday in arguably the biggest match of both teams' seasons so far.
Consequently, the Argentine says that he will wait until tomorrow before making a decision on Alli.
"Dele Alli had problems before & during the game. He’s OK but we’ll assess tomorrow & decide," the Lilywhites' Twitter page reports him as saying.
Alli has undoubtedly been one of Spurs' stand-out players in what has been a magnificent season at White Hart Lane on an individual and collective level, and losing him for either match this week could be a huge blow.