The Reds picked up further hamstring injuries in the 1-0 win over Stoke.
The German has been plagued by a number of injuries since taking charge at Anfield, with a number of players currently on the sidelines.
Tuesday’s cup clash with Stoke saw another two players join that list, with Philippe Coutinho and Dejan Lovren forced off before half time with hamstring injuries.
In fact, of Liverpool’s current tally of eight injuries, five are hamstring issues, and Souness doesn’t believe that the injuries are a coincidence but a result of Klopp’s preferred style of play.
"A new manager comes in and all the chat was about high press further up the field. It's hard work to do that for 90 minutes,” he told Sky Sports.
“You can do it in bursts. You can do it for an hour, you can do it for 70 minutes, but to do it for 90 minutes is a big demand on your legs.”
"I just think they've fallen foul of that. It's not a coincidence, five hamstrings. To have them all at one time, I think they have to look at what they're doing in training."
While it had seemed that Klopp would be quiet in the January transfer window, the injury to Lovren may end up changing that stance.
With Martin Skrtel and Mahmadou Sakho also both out, and Joe Gomez a long-term absentee, he is seriously limited in his options at the back.
Kolo Toure was also visibly struggling towards the end of the game at the Britannia, and Klopp may be tempted to add to his squad to ensure a crisis is avoided in the second half of the campaign.