Jackett’s side suffered a 3-2 defeat to the Whites at Molineux in the Championship despite taking the lead after just 10 minutes.
Leeds were the better side in the second half and deservedly picked up all three points.
“We were in control of the game and then their substitution worked for them,” Jackett told Wolves’ official website. “I understand it was an injury but the change in formation saw them out-power us in the last 15 minutes of the first half and then particularly in the start of the second.
“They got a lot of shots in and had one or two chances even before they did score and Carl Ikeme made two good saves.
“I thought they got on top of us physically and I didn’t think in that period that we were strong enough or our defending was good enough. They had too much strength for us and too much aggression, they won the game in that period.”
Over the course of the 90 minutes at Molineux, hosts Wolves had 50% of the possession, took 10 shots of which just three were on target, and earned six corners.
Visitors Leeds had 50% of the possession, took 14 shots of which only six were on target, and earned two corners.
Wolves are 17th in the Championship table at the moment with 25 points from 21 matches, while Leeds are a point ahead of them in 14th.
Both sides’ chances of making it through to the playoffs are still there, but it will be tough for them.