Agbonlahor was left out of Villa’s match-day squad to face Arsenal last weekend, with Garde preferring other options ahead of him.
And the French boss admits that there will be certain members of his squad who still need time to adapt to his managerial methods.
“When a player is not selected in the team, like Gabby last week, obviously it creates a disagreement,” he said.
"Sometimes disagreements or mistakes in life are not definitive.
"Sometimes you learn from that. You can have a discussion and have a new start. It can match or it can not match, I don't know.
"For Gabby and the rest of the team, when you change manager and the way of working, maybe suddenly you don't feel comfortable.
"I'm not saying that about Gabby especially. It will be the same for every player.”
As a result of Agbonalhor’s omission against Arsenal, speculation has started about the striker’s future at the club.
The Daily Mail suggest that Agbonlahor could be sold in January, with Norwich City one of the clubs said to be interested.
The 29-year-old has spent his entire career with the Villans to date, but it seems that he will need to find a new home if he is to secure regular first-team football now.
Agbonlahor has played 10 times for Villa so far this season, but he is yet to hit the back of the net.