The John Lyall gates will be uprooted and relocated inside the club shop at the 54,000 seater stadium on Queen Elizabeth Park.
The gates, which have stood proudly outside the club's home since 1956, were renamed in Lyall's honour in 2009 after he had died three years before aged 66.
After a playing career cruelly cut short by injury, Lyall joined the club’s coaching ranks in the 1960s under Ron Greenwood. He went on to succeed his mentor as manager in 1974, leading the Hammers with distinction and continuing the evolution of the ‘West Ham Way’.
But his legacy will now live on at the Premier League side's new home with supporters able to visit the gates for pictures inside the stadium store.
There they will stand as a tribute to one of the club’s greatest servants and most successful managers, who twice brought the FA Cup to West Ham in 1975 and 1980 and led the club to its last European final in the 1976 Cup Winners’ Cup.
It may not be the only thing West Ham bring with them down the road to Stratford, though. There are also plans to relocate the Champions Statue, the West Ham Pals Battalion Memorial Plaque, and the other commemorative bricks and plaques outside Upton Park.
Lyall's son Murray told the club's official website he is delighted his dad will be immortalised at the new ground.
“We’re honoured that Karren Brady has delivered on her promise to take the John Lyall Gates with us to the new stadium,” he said.
“That means so much to us as a family.
“Dad left a great legacy at Upton Park and the gates were a testimony to that. I think he’d have been thrilled about the move."
The club's vice-chairman Karren Brady said the stadium should look and feel like the home of West Ham United, both inside and out.
"We have listened to the feedback from supporters that told us how important the John Lyall Gates were to them, so I am delighted that we will now be taking one of the most striking features of the Boleyn Ground with us," she said.
“A number of other iconic features will also make the journey with us to Stratford as we are committed to honouring our rich history and club legends at our new home.”
The Hammers will take up residency of the Olympic Stadium in time for the start of the 2016/17 season.