Larsson is currently suspended after picking up 10 yellow cards for the season and is gutted that he cannot play a part in Sunderland's survival fight this weekend against Stoke City.
The Swedish midfielder has trained as if he is 'desperate' to play this week, according to Advocaat, but the experienced Dutch boss has no choice but to leave him on the sidelines on Saturday.
"Larsson shows on the training pitch that he is desperate to start again," Advocaat said.
It is a blow for Sunderland to be without Larsson as he is one of their most talented midfield players and could be crucial to their chances of staying up.
Larsson offers a calming influence in attacking situations, while also boasting a combative streak which makes him a key part of their starting line-up.
Without him in the team, Advocaat's options in the middle of the park look limited, and that could be costly with the side facing a tough test at the Britannia Stadium.
Sunderland are currently just one point clear of the Premier League drop zone and will be determined to pick up a vital victory tomorrow.