The Spanish national team coach was not happy with the recent comments from the Chelsea manager.
Ahead of the most recent international break Chelsea striker Diego Costa was left out the Spain squad, after returning from his previous call-up a wounded soldier - angering Mourinho and the Chelsea hierarchy.
This time around they gave Costa a chance to rest, but then Cesc Fabregas pulled out, leaving many to come up with their own conspiracy theories and for the Spanish press to bash Chelsea for applying pressure to their players.
This week Mourinho was again stoking this fire. In reply to insinuations about the fitness of Costa and Fabregas from Sergio Ramos and Del Bosque.
The Chelsea boss was reported to had said:
"Since I've known Sergio Ramos he has been a fantastic football player, but he is not a doctor. He is not a doctor and I am the same. I do my job the best I can but I am not a doctor.
"My doctor and the doctor of the Spanish national team, they had the scans and they decided that the player was not in the condition to play.
"I am nobody to go against it and I don't think Sergio did a medicine Masters in the last couple of years to understand about it.
"I trust my doctor 100 per cent. If Del Bosque doesn't trust his doctor that is his problem, not my problem. I speak with my doctor and I believe in what my doctor tells me. Nothing else. No problem,"
Now, Del Bosque has bit back.
"I have nothing to say. We absolutely appreciate what a great coach Mourinho is, but I think people should talk about things closer to home.
"People should stick to what concerns them, to their jobs, their work and nothing else."
This schoolyard argument is likely to continue as long as both men stay in their respective jobs - so for quite a while yet.