His club will be hoping the injury he picked up in training isn't too serious to their first-choice left-back.
Arsenal's Kieran Gibbs is expected to deputise in the absence of Baines as he is England's only recognised left-back still with the squad.
The Toffees will be hoping Baines is fit enough to face West Ham United at Goodison Park on November 22 as Everton will look to build on a recent good run which has seen them on the brink of qualifying for the knockout stages of the Europa League, and into the top half of the Premier League table.
A statement on the Everton website said the 29-year-old will return to the club's Finch Farm training ground for assessment but there has been no word on the precise nature of the injury so far.
It's the second notable injury blow for the club during this international break with James McCarthy withdrawing from the Republic of Ireland squad because of a hamstring problem - though the injury itself was sustained during the 1-1 draw with Sunderland prior to him linking up with his national side.
Baines has scored three goals for the club this season and provided nine assists, which demonstrates the contribution he makes for Roberto Martinez's side going forward as well as in defence.