Chalobah completed a switch to Turf Moor at the end of the transfer window, joining up with the former Championship side until the beginning of January.
The 19-year-old midfielder was still in the process of returning to match fitness when he made his debut for the Clarets last weekend, coming on as a half-time substitute as Burnley went down 4-0 to West Bromwich Albion.
With a number of midfielders currently on the operating table, with Dean Marney, David Jones and Matt Taylor all missing the West Brom defeat, Chalobah was rushed into action, and he had a solid, if not inspiring game.
With it looking increasingly likely that the England Under-21 international would be the only senior central midfielder available for Saturday’s clash with Leicester City, Chalobah was used in an Under-21 Premier League tie to encourage his return to match fitness - although he left the field early after a rough challenge.
The midfielder was clattered by an opposing player, and, having been taken to hospital, it has been revealed that he suffered an injury to his windpipe. Although not overly threatening, Clarets boss Sean Dyche confirmed to the Burnley Express that Chalobah will not be fit for the trip to the King Power Stadium.
He said: “Nathaniel won’t be fit. He hurt his windpipe on Tuesday after an arm to the throat, and there is some damage there.
“He’s not in any danger, but we can’t put it at further risk of getting another whack.
“We’ll have it checked again once the swelling settles down, as it can effect your breathing.
“It’s unfortunate, but he comes out of the midfield as well, so we’re going to have to be flexible again.”
With the number of midfielders currently out of action, Dyche could be forced to field a squad of unproven or out-of-position players. However, he did confirm that Marney and Jones are on their way back, although there is no guarantees for when they will return.
Dyche added: “They are both 50/50 at best at the moment, touch and go. They’ve improved, but there’s a difference between improving and being ready to play a Premier League game."