The Canadian chief, 54, bullishly claimed that neither Jay Rodriguez or Morgan Schneiderlin will be permitted to complete transfers out of Southampton - whether they want to stay at the club or not.
Speaking to talkSPORT on Tuesday, the former NHL coach-turned-chairman said that the club had "reached a tipping point", meaning that the exodus of players must now be halted.
Southampton have lost no less than five regular faces that featured in last season's 18-man match-day squads.
Krueger however, remained adamant that the two in-demand players, who are both wanted by former boss Mauricio Pochettino at Tottenham, would not be allowed to leave the club.
"I guarantee they will be here come September. September is judgement day and people need to realise we are in a process, the match is far from over."
Whilst Jay Rodriguez has made no public reply to the comments made by Kreuger, Morgan Schneiderlin has already hit out at the Canadian's decision, by tweeting:
6 years of an amazing journey #saintsfc DESTROYED in 1 hour !!!— Morgan Schneiderlin (@SchneiderlinMo4) July 29, 2014
Schneiderlin's tweet refers to a meeting held with club supremos Les Reed and Ralph Krueger on Tuesday, with both club directors refusing to permit Schneiderlin his desired move to Tottenham.
The Frenchman is seen as a key component of Ronald Koeman's plans for next season, with the Dutchman reiterating in his opening press conference: "Schneiderlin is a Southampton player."
It appears that Southampton are now wishing to draw a line in the sand and say 'no' to any further departures, as Koeman prepares to rebuild the first team ahead of the new season.
Southampton have not responded to the comments made by Schneiderlin, who it appears will be expected to carry on with life at St. Mary's for the foreseeable future.