The five-time winners continue their World Cup campaign in Fortaleza against their fellow South Americans, and victory will all but assure their qualification to the second phase.
Scolari's side kicked off things with a 2-1 win against Croatia last Thursday, and the former Chelsea manager has said that the Mexicans are a side that Selecao cannot take lightly on Tuesday.
FIFA.com quotes Scolari as saying: "Every time we play against Mexico we have difficulties. That is why it is a game the players know will be very even, they need to have very good conditioning from the beginning until the end.
"The two teams play football of very good quality, with the ball on the ground, lots of moving around. That's why we will insist on all the necessary training, on the necessary respect towards Mexico. But we know a victory will put us in a highly comfortable position for the next round, so we'll play with that in mind."
Meanwhile, Chelsea playmaker Oscar agreed with his manager's assertion that their opponents shouldn't be underestimated.
"It will certainly be as difficult as last year," said Oscar. "In the World Cup there is an extra desire to win and I hope we can do so again this time.
"Mexico are a very different side to Croatia. They play the ball out from the back well and are very good on the counter-attack."
Brazil could be without Hulk for the game after the Zenit St Petersburg striker suffered a hamstring injury.